VEGF164-2

Yeast

Vascular Endothelial Growth Factor Isoform 164

Vascular endothelial growth factor (VEGF) is a highly specific mitogen for vascular endothelial cells. Five VEGF isoforms are generated as a result of alternative splicing from a single VEGF gene. These isoforms differ in their molecular mass and in biological properties such as their ability to bind to cell-surface heparan-sulfate proteoglycans. Mouse VEGF164 shares 97% aa sequence identity with corresponding regions of rat, 89% with human and porcine, 88% with bovine, and 90% with feline, equine and canine VEGF, respectively. During pathological neovascularization, both the absolute and relative expression levels for VEGF164 increased to a greater degree than during physiological neovascularization.

Approximately 25.7 kDa in SDS-PAGE under non-reducing conditions.

MAPTTEGEQK AHEVVKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNVTMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR

Sterile Filtered White lyophilized (freeze-dried) powder.

> 95% by SDS-PAGE and 90% by SEC-HPLC analyses.

Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 0.753.75 ng/mL. Fully biologically active when compared to standard.

< 0.1 EU per 1μg of the protein

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.