5'-Cholesteryl-Triethylene Glycol-CE Phosphoramidite

10-O-[1-propyl-N3-carbamoylcholesteryl]-triethyleneglycol-1-[(2-cyanoethyl)-(N, N-diisopropyl)]-phosphoramidite

For 5'-attachment, we have found 5'-Cholesterol-CE Phosphoramidite (LK2170) to offer specific advantages in oligo synthesis. Since the cholesterol is attached directly to aminohexanol, it is not susceptible to 1,2-diol elimination as observed in some other products. Lack of a trityl group simplifies purification; some cholesterol products must be used in trityl-on mode (to prevent 1,2-diol elimination during deprotection), then detritylated, and can subsequently be very difficult to purify. Similarly, 5'-Cholesterol-TEG-CE Phosphoramidite (LK2189) is a simple 5'-modifier without the complications of a 1,2-diol and trityl protection, but with the added benefit of solubility in acetonitrile conferred by the TEG spacer.

C46H82N3O7P

820.15

Colorless to light yellow oil