UltraNuclease GMP-grade

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Cat Number

PIPB-0794

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Product Detail Description Customer Q&A

Storage

Store at -20 ℃

Molecular Weight

26.5 kDa

Appearance

Clear and transparent liquid

Standard

Facility GMP

Qualification

DMF

General Description

UltraNuclease is an extremely pure, recombinant endonuclease protein that is originally from Serratia marcescens, produced in Escherichia coli (E. coli), made in a cGMP facility, contains no animal-derived ingredients and is available in low endotoxin form. UltraNuclease is preferred by biomanufacturers due to its high activity and broad utility.

Mechanism of Action

UltraNuclease is a non-specific endonuclease that cleaves the phosphodiester bonds within the nucleic acid backbone. UltraNuclease completely degrades all types of DNA and RNA, including double- and single-stranded, linear and circular, to produce small (3-5 base) oligonucleotides with 5'-monophosphate ends. This level of complete degradation allows UltraNuclease to be used to effectively remove nucleic acid impurities during purification.

Application

UltraNuclease is an essential reagent for the removal of surface- and residual DNA/RNA during large-scale biomanufacturing processes and research. The most common application is to reduce the viscosity of cell supernatants and lysates, which can increase the efficiency of protein purification many-folds. UltraNuclease is also employed in sample preparation for electrophoresis/chromatography and to inhibit the clumping of stored peripheral blood monocytic cells.

What is the primary use of UltraNuclease?

UltraNuclease is primarily used for purifying biological products from nucleic acid contaminants.

Which nucleic acids does UltraNuclease degrade?

UltraNuclease degrades all forms of DNA and RNA, including single-stranded and double-stranded, linear and circular molecules.

Is your UltraNuclease animal-origin free?

Yes, UltraNuclease is made through recombinant expression in E. coli and contains no animal-derived components.

Can I use UltraNuclease for vaccine manufacturing?

Yes, UltraNuclease is commonly used to purify viral vaccines and oncolytic viruses by removing host cell nucleic acids.