mRNA Cap 2\'-O-Methyltransferase GMP-grade

Inquiry

Cat Number

PIPB-0796

If you have any other questions, please contact our experts.

Product Detail Description Customer Q&A

Storage

Store at -20 ℃

Appearance

Clear and transparent liquid

Standard

Facility GMP

Qualification

DMF

General Description

mRNA Cap 2'-O-Methyltransferase is a recombinant enzyme, from vaccinia virus, produced according to GMP standards and is supplied in a liquid format. The enzyme is free of antibiotics as well as animal-derived components. It is of high purity and designed to modify the 5'-end of mRNA transcripts to generate the Cap1 structure.

Mechanism of Action

mRNA Cap 2'-O-Methyltransferase is a methyltransferase that uses S-adenosylmethionine (SAM) as the methyl donor and is used to methylate capped RNA. It can act on RNA molecules that already contain the 7GpppN cap (Cap0) and transfer a methyl group to the 2'-O position of the first nucleotide adjacent to the m7G cap, thus, converting the Cap0 to the higher-order Cap1 structure. This Cap1 structure is more widely used in high eukaryotes and is important for innate immune evasion.

Application

mRNA Cap 2'-O-Methyltransferase is used for the production of mRNA therapeutics and vaccines, for which the resulting Cap1 structure is important for both enhanced expression during transfection or microinjection studies and to enable the mRNA to evade innate immune sensing in vivo. It is also used for RNA modification and functional studies in the research setting.