Cat Number: GMP-101-03

rHuIL-3 GMP

Inquiry

Source

Escherichia coli

Purity

> 98% by SDS-PAGE and HPLC analyses.

Molecular Weight

Approximately 15.0 kDa, a single non-glycosylated polypeptide chain containing 133 amino acids.

Sequence

APMTQTTSLK TSWVNCSNMI DEIITHLKQP PLPLLDFNNL NGEDQDILME NNLRRPNLEA FNRAVKSLQN ASAIESILKN LLPCLPLATA APTRHPIHIK DGDWNEFRRK LTFYLKTLEN AQAQQTTLSL AIF

Biological Activity

> 1.0 × 10⁷ U/mg

Appearance

Sterile filtered white lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2 µm filtered concentrated solution in PBS, 0.02% Tween-20, pH 7.0.

Storage

Store at -15℃ to -20℃

Shipping

The product is shipped with polar packs.

Endotoxin

Less than 0.01 EU/μg

Host Cell Protein

Less than 0.05%

Host Cell DNA

Less than 20 ng/mg

Reconstitution

Prior to opening, it is recommended to centrifuge the vial briefly to bring the contents down the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. If animal-origin-free condition is expected in your product, then sterile distilled water is recommended. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Usage

For research or further manufacturing use only, not for direct human use.

Sterility

Conforms

Mycoplasma

Negative

In Vitro Virus Assay

Negative

Background

Interleukin-3 (IL-3) is an interleukin, a type of biological signal (cytokine) which is encoded by the IL3 gene located on chromosome 5 and produced primarily by activated T cells beside human thymic epithelial cells, activated murine mast cells, murine keratinocytes, and neurons/astrocytes. The protein acts in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes, and the monocytes-macrophages. The human IL-3 reported to be a monomer, as it is known, contains 133 amino acids residues which is a single non-glycosylated polypeptide. Specifically, human, and murine IL-3 share low homology and it does not show activity on murine cells.