Cat Number: GMP-101-04

rHuIL-4 GMP

Inquiry

Source

Escherichia coli

Purity

> 98% by SDS-PAGE analyses.
> 95% by HPLC analyses.

Molecular Weight

Approximately 15.0 kDa, a single non-glycosylated polypeptide chain containing 129 amino acids.

Sequence

HKCDITLQEI IKTLNSLTEQ KTLCTELTVT DIFAASKNTT EKETFCRAAT VLRQFYSHHE KDTRCLGATA QQFHRHKQLI RFLKRLDRNL WGLAGLNSCP VKEANQSTLE NFLERLKTIM REKYSKCSS

Biological Activity

> 5.0 × 10⁶ IU/mg

Appearance

Sterile filtered white lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2 µm filtered concentrated solution in PBS, 0.02% Tween-20, pH 5.0.

Storage

Store at -15℃ to -20℃

Shipping

The product is shipped with polar packs.

Endotoxin

Less than 0.01 EU/μg

Host Cell Protein

Less than 0.05%

Host Cell DNA

Less than 20 ng/mg

Reconstitution

Prior to opening, it is recommended to centrifuge the vial briefly to bring the contents down the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. If animal-origin-free condition is expected in your product, then sterile distilled water is recommended. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Usage

For research or further manufacturing use only, not for direct human use.

Sterility

Conforms

Mycoplasma

Negative

In Vitro Virus Assay

Negative

Background

Interleukin-4 (IL-4) is a pleiotropic cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells and produced by mast cells, activated T cells and bone marrow stromal cells. It has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of CD4+ T-cells into Th2 cells. In addition, it enhances both secretion and cell surface expression of IgE and IgG1 and also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. The human IL-4 has a compact, globular fold, stabilized by 3 disulphide bonds. The human IL-4 shares about 40% aa sequence identity with mouse/rat IL-4 and they are species-specific in their activities.