rHuIGF-1 GMP

Escherichia coli

> 98% by SDS-PAGE and HPLC analyses.

Approximately 7.6 kDa, a single non-glycosylated polypeptide chain containing 70 amino acids.

GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA

>1000 IU/mg

Sterile filtered white lyophilized (freeze-dried) powder.

Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.0.

Store at -15℃ to -20℃

The product is shipped with polar packs.

Less than 0.01 EU/μg

Less than 0.05%

Less than 20 ng/mg

Prior to opening, it is recommended to centrifuge the vial briefly to bring the contents down the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. If animal-origin-free condition is expected in your product, then sterile distilled water is recommended. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

For research or further manufacturing use only, not for direct human use.

Conforms

Negative

Negative

The insulin-like growth factors (IGFs) belonged to the insulin gene family, are mitogenic polypeptide growth factors that stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. The IGFs are similar by structure and function to insulin, but have a much higher growth-promoting activity than insulin. IGF-1 is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. The production of IGF-1 is stimulated by growth hormone (GH) and can be retarded by undernutrition, growth hormone insensitivity, lack of growth hormone receptors, or failures of the downstream signaling pathway post GH receptor including SHP2 and STAT5B. Recombinant human IGF-1 are globular proteins containing 70 amino acids and 3 intra-molecular disulfide bonds. Mature human IGF-1 shares 94% and 96% a.a. sequence identity with mouse and rat IGF-1, respectively, and exhibits cross-species activity.