Flt-3 Ligand-1

E.coli

Flt3-Ligand, FL, FLG3L, Flt3L, SL Cytokine

The cytokine Fms-like tyrosine kinase 3 ligand (FLT3L), also known as Flt-3 Ligand, is an important regulator of hematopoiesis. Its receptor, Flt3, is expressed on myeloid, lymphoid and dendritic cell progenitors and is considered an important growth and differentiation factor for several hematopoietic lineages. Activating mutations of Flt3 are frequently found in acute myeloid leukemia (AML) patients and associated with a poor clinical prognosis. Mature human Flt_x005f3L consists of a 158 amino acid (aa) extracellular domain (ECD) with a cytokine-like domain and a juxtamembrane tether region, a 21 aa transmembrane segment, and a 30 aa cytoplasmic tail. At the amino acid sequence level, human and mouse FL are approximately 72% identical and the two proteins exhibit cross-species activity. The Flt3-L manifests antitumor activity, presumably due to its capacity to recruit dendritic cells and cause their proliferation.

Approximately 17.6 kDa.

TQDCSFQHSP ISSDFAVKIR ELSDYLLQDY PVTVASNLQD EELCGGLWRL VLAQRWMERL KTVAGSKMQG LLERVNTEIH FVTKCAFQPP PSCLRFVQTN ISRLLQETSE QLVALKPWIT RQNFSRCLEL QCQPDSSTLP PPWSPRPLEA TAPTA

Sterile Filtered White lyophilized (freeze-dried) powder.

> 97% by SDS-PAGE and HPLC analyses.

The ED50 as determined by a cell proliferation assay using human AML5 cells is less than 1.0 ng/mL, corresponding to a specific activity of > 1.0 × 106 IU/mg. Fully biologically active when compared to standard.

< 1.0 EU per 1μg of the protein

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.0.