BspQl GMP-grade

BspQI is an optimal Type IIS restriction endonuclease with high efficiency and low star activity, produced under GMP conditions and tested free of endonuclease, exonuclease and RNase activities. It is a recombinant protein expressed in E. coli. The uniform quality and activity of BspQI make it a reliable reagent for preserving sample integrity in critical molecular biology workflows.

BspQI can recognize the asymmetric 5'-GCTCTTCN1/N4-3' sequence and cuts the double stranded DNA outside of its recognition site. This creates linearized DNA fragments with defined cohesive ends for downstream applications. This property of the BspQI restriction enzyme allows for excision of DNA at a defined distance from a specific recognition site and the creation of a defined overhang.

BspQI is used in large-scale high-throughput and automated molecular cloning workflows. It is primarily used for plasmid digestion in order to linearize plasmids as templates for applications like in vitro transcription. Linearized DNA fragments with defined poly(A/T/G/C)-terminated cohesive ends are also produced for downstream manipulation. BspQI is also used in general restriction digest applications, molecular cloning, restriction site mapping, genotyping, and SNP analysis.