Deoxyribonuclease I (DNase I) GMP-grade

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Cat Number

PIPB-0800

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Product Detail Description Scientific Support Customer Q&A

Storage

Store at -20 ℃

Synonyms

DNase I

Standard

Facility GMP

Qualification

DMF

General Description

Deoxyribonuclease I (DNase I) has highly specific and robust DNA hydrolyzing activity and is produced under strict GMP process controls to support the stringent requirements of mRNA therapeutics and vaccine manufacturers. This liquid formulation is extensively purified to remove any RNase activity, allowing for the quality and stability of RNA samples. DNase I can degrade all forms of DNA including single- and double-stranded DNA as well as chromatin and is therefore widely used to digest DNA from samples to provide pure, undegraded RNA for downstream applications.

Mechanism of Action

DNase I is an endonuclease that hydrolyzes the phosphodiester bond within DNA, yielding short oligodeoxynucleotides (down to polytetranucleotides) with 5'-phosphate and 3'-OH ends.

Application

DNase I is widely used in the efficient digestion of contaminated DNA from RNA samples for RNA purification and sample quality control prior to RNA-based assays such as RT-PCR and RT-qPCR. DNase I is also commonly used to digest any residual DNA templates after the in vitro transcription reaction.

A free molecular beacon (MB), a short DNA probe, is quickly and quantitatively digested by DNase I in vitro within 15 minutes. To protect the MB from degradation, the authors adsorbed the MB onto a nanoscale graphene oxide (NGO) sheet through π-stacking interactions. The NGO sheet sterically shields the DNA probe by physically excluding the bulky DNase I enzyme from the DNA backbone of the MB. This protection is crucial to safely deliver the probe into a nuclease-rich cellular environment, where nucleases such as DNase I are abundant. In HeLa cells, the NGO-MB complex's protection remains. Confocal microscopy and flow cytometry show bright Cy5 fluorescence only when survivin mRNA is present, while the scrambled sequence control remains dark.

Fig. 1 NGO delivery of MB into HeLa cells to detect survivin mRNA. (Lu C H.; et al. 2010)

References

Lu C H, et al. Using graphene to protect DNA from cleavage during cellular delivery. Chemical Communications, 2010, 46(18): 3116-3118.

What type of enzyme is DNase I?

DNase I is an endonuclease that cleaves single-stranded and double-stranded DNA.

Is your DNase I compatible with mRNA vaccine production?

Yes, our GMP Grade DNase I is designed and optimized for mRNA therapeutics and vaccine manufacturing.

How does DNase I function in RNA purification?

It degrades contaminated DNA in RNA purification workflows, ensuring that the final RNA sample is free of DNA.

Does your DNase I contain any animal-origin materials or antibiotics?

No, our DNase I is manufactured under GMP guidelines without antibiotics and is animal-origin free.