Cat Number: 92517ES

Amphiregulin

Source

E.coli

Synonyms

AR, AREG, Colorectum Cell-derived Growth Factor, CRDGF

Description

Amphiregulin is an EGF related growth factor that signals through the EGF/TGF-α receptor, and stimulates growth of keratinocytes, epithelial cells and some fibroblasts. Amphiregulin also inhibits the growth of certain carcinoma cell lines. Synthesized as a transmembrane protein, Amphiregulin’s extracellular domain is proteolytically processed to release the mature protein. There are 6 conserved cysteine residues, which form 3 intramolecular disulfide bonds essential for biological activity. Recombinant Human Amphiregulin is an 11.3 kDa glycoprotein consisting of 98 amino acid residues.

Molecular Weight

Approximately 11.3 kDa.

AA sequence

SVRVEQVVKP PQNKTESENT SDKPKRKKKG GKNGKNRRNR KKKNPCNAEF QNFCIHGECK YIEHLEAVTC KCQQEYFGER CGEKSMKTHS MIDSSLSK

Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

> 95 % by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is between 5-10 ng/mL.

Endotoxin

< 1.0 EU per 1μg of the protein

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4.

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Sunday : 9.30 AM–1.00 PM
Holidays: Closed

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