Cat Number: GMP-103-01

rHuTNF-α/TNFSF2 GMP

Inquiry

Source

Escherichia coli

Purity

> 98% by SDS-PAGE and HPLC analyses.

Molecular Weight

Approximately 17.5 kDa, a single non-glycosylated polypeptide chain containing 158 amino acids.

Sequence

MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL

Biological Activity

> 2.0 × 10⁷ IU/mg

Appearance

Sterile filtered white lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in 5 mM Na2HPO4, 5 mM NaH2PO4, 10 mM NaCl, pH 7.0.

Storage

Store at -15℃ to -20℃

Shipping

The product is shipped with polar packs.

Endotoxin

Less than 0.01 EU/μg

Host Cell Protein

Less than 0.05%

Host Cell DNA

Less than 20 ng/mg

Reconstitution

Prior to opening, it is recommended to centrifuge the vial briefly to bring the contents down the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. If animal-origin-free condition is expected in your product, then sterile distilled water is recommended. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Usage

For research or further manufacturing use only, not for direct human use.

Sterility

Conforms

Mycoplasma

Negative

In Vitro Virus Assay

Negative

Background

Tumor necrosis factor alpha (TNF-α), also called cachectin, is the best-know member of the TNF-family, which can cause cell death. This protein is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. TNF-α occurs as a secreted, soluble form and as a membrane-anchored form, both of which are biologically active. The naturally-occurring form of TNF-α is glycosylated, but non-glycosylated recombinant TNF-α has comparable biological activity. The biologically active native form of TNF-α is reportedly a trimer. Human and murine TNF-α show approximately 79% homology at the amino acid level and cross-reactivity between the two species. Two types of receptors for TNF-α have been described and virtually all cell types studied show the presence of one or both of these receptor types.