VEGF120-1

Yeast

Vascular endothelial growth factor isoform 120

Vascular endothelial growth factor (VEGF) is a highly specific mitogen for vascular endothelial cells. Five VEGF isoforms are generated as a result of alternative splicing from a single VEGF gene. These isoforms differ in their molecular mass and in biological properties such as their ability to bind to cell-surface heparan-sulfate proteoglycans. Mouse VEGF120 shares 98% aa sequence identity with corresponding regions of rat, 89% with canine, feline, equine and porcine, and 87% with human, ovine and bovine VEGF, respectively. VEGF120 and VEGF164, for inducing leukocyte stasis (leukostasis) within the retinal vasculature and blood-retinal barrier (BRB) breakdown and to determine whether endogenous VEGF164 mediates retinal leukostasis.

Approximately 20.7 kDa in SDS-PAGE under reducing conditions.

MAPTTEGEQK SHEVIKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNITMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKCDKPR R

Sterile Filtered White lyophilized (freeze-dried) powder.

> 95% by SDS-PAGE and 90% by SEC-HPLC analyses.

Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 1-4 ng/mL. Fully biologically active when compared to standard.

< 0.1 EU per 1μg of the protein

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.