Cat Number: 91705ES

TGF-β3

Genus

Human

Source

E.coli(His-tagged)

Synonyms

TGF-β3; ARVD; ARVD1; FLJ16571; LDS5; RNHF; TGFB3; TGFbeta 3; TGF-beta 3; TGF-beta3; TGF-beta-3; transforming growth factor beta-3; transforming growth factor beta 3

Description

TGFβ3 is a member of the TGF-β superfamily subgroup defined by its structural and functional similarities.TGF-β3 and β1, β2 act as cellular switches to regulate immune function, cell proliferation and epithelial-mesenchymal transition. The human TGF-β3 cDNA encodes the 412 aa precursor, which contains 20 aa signaling peptides and 392 aa proteins. Mature human TGF-β3 shares 100%, 99%, and 98% homology with mouse/dog/horse, rat, and porcine TGF-β3, respectively.TGF-β3 has high affinity for TGF-βRII, a type II serine/threonine kinase receptor. This receptor phosphorylates and activates the type I serine/threonine kinase receptor TGF-βRI or ALK-1 to regulate transcription via Smad phosphorylation.

Molecular Weight

Approximately 12 kDa.

AA sequence

MALDTNYCFRN LEENCCVRPL YIDFRQDLGW KWVHEPKGYY ANFCSGPCPY LRSADTTHST VLGLYNTLNP EASASPCCVP QDLEPLTILY YVGRTPKVEQ LSNMVVKSCK CS

Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

> 98% as determined by SDS-PAGE.

Biological Activity

Measure by its ability to inhibit IL- 4-induce proliferation in HT-2 cells. The ED50 for this effect is <50 pg/mL. The specific activity of recombinant human TGF beta 3 is > 2 x 107 IU/mg.

Endotoxin

< 0.1 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in 1×PBS, pH3.5.

Note

5, 100μg, 1mg