Salt Active UltraNuclease GMP-grade

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Cat Number

PIPB-0801

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Product Detail Description Scientific Support Customer Q&A

Storage

Store at -20 ℃

Molecular Weight

26.5 kDa

Standard

Facility GMP

Qualification

DMF

General Description

UltraNuclease is a recombinant, high-purity endonuclease, which is manufactured in GMP facilities and produced without animal-derived or antibiotic components. UltraNuclease is exclusively a Salt Active Nuclease, and designed to function optimally in high salt concentrations. These characteristics, combined with its overall high salt tolerance, make UltraNuclease suitable for laboratory research as well as high containment to large scale biomanufacturing that require high stringency and control of host nucleic acid residues.

Mechanism of Action

UltraNuclease cleaves the phosphodiester bonds that form the backbone of nucleic acids, and can efficiently degrade various types of DNA and RNA (ssRNA, dsRNA, ssDNA, dsDNA, linear or circular) into small oligonucleotides of approximately 5 bp in length. The high salt tolerance and salt active mechanism are particularly important for the nuclease to dissociate and degrade nucleic acids that have been released from proteins or other cellular constituents in the high salt buffers that are typically used to reduce protein aggregation during the purification process.

Application

UltraNuclease is utilized for the purification of viral vaccines, viral vectors, and oncolytic viruses, as well as protein and polysaccharide pharmaceuticals. It is also used more broadly to reduce solution viscosity in cell supernatants and lysates, which results in an overall improvement in protein purification yields, and for sample preparation of analytical methods such as electrophoresis and chromatography.

The small-molecule Z9 (chromone–dithiocarbamate conjugate) inhibits tomato-spotted-wilt-virus (TSWV) replication by disrupting TSWV phase-separated N-protein–RNA condensates. Z9 binds to N's nucleic-acid-binding pocket, inhibits its RNA-induced condensation, and promotes N degradation. In planta, Z9 reduces inclusion-body numbers >50%, lowers viral RNP fluorescence and systemic infection by 80%. Key mechanistic evidence was obtained with UltraNuclease: when total RNA in N-expressing leaf extracts was enzymatically hydrolyzed, N protein levels fell as sharply as with Z9 treatment, proving that RNA within condensates normally shields N from proteolysis. Thus, Z9 pioneers condensate-targeting antivirals for crops and UltraNuclease served as the decisive tool to demonstrate RNA-dependent stabilization of the viral nucleocapsid.

Fig. 1 UltraNuclease hydrolyzes RNA in N-expressing leaves. (Zan N.; et al. 2025)

References

Zan N, et al. Rational design of phytovirucide inhibiting nucleocapsid protein aggregation in tomato spotted wilt virus. Nature Communications, 2025, 16(1): 2034.

Where can Salt Active UltraNuclease be purchased?

Salt Active UltraNuclease is available in all major markets, including North America, Europe and Asia.

Can Salt Active UltraNuclease be used in vaccine manufacturing?

Yes, Salt Active UltraNuclease is well-suited to purifying viral vaccines, vectors and oncolytic viruses.

Can your UltraNuclease be used in high salt?

Yes, unlike many other nucleases our UltraNuclease has very high activity even at high salt concentrations, making it useful in difficult purifications.

How can Salt Active UltraNuclease enhance protein purification?

Salt Active UltraNuclease can reduce viscosity by degrading nucleic acids, increasing clarification efficiency and downstream purification yields.

What forms of nucleic acids can Salt Active UltraNuclease degrade?

Salt Active UltraNuclease nonspecifically degrades DNA and RNA, in its single-stranded, double-stranded, linear and circular forms.