rHuKGF-1/FGF-7 GMP

Escherichia coli

> 98% by SDS-PAGE and HPLC analyses.

Approximately 18.9 kDa, a single, non-glycosylated polypeptide chain containing 163 amino acids.

CNDMTPEQMA TNVNCSSPER HTRSYDYMEG GDIRVRRLFC RTQWYLRIDK RGKVKGTQEM KNNYNIMEIR TVAVGIVAIK GVESEFYLAM NKEGKLYAKK ECNEDCNFKE LILENHYNTY ASAKWTHNGG EMFVALNQKG IPVRGKKTKK EQKTAHFLPM AIT

> 1.0 × 10⁵ U/mg

Sterile filtered white lyophilized (freeze-dried) powder.

Lyophilized from a 0.2 µm filtered solution in 20 mM PB, 500 mM NaCl, 5% Trehalose, 0.02% Tween-20, pH 7.4.

Store at -15℃ to -20℃

The product is shipped with polar packs.

Less than 0.01 EU/μg

Less than 0.10%

Prior to opening, it is recommended to centrifuge the vial briefly to bring the contents down the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. If animal-origin-free condition is expected in your product, then sterile distilled water is recommended. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

For research or further manufacturing use only, not for direct human use.

Human KGF-1 also known as Fibroblast growth factor 7 (FGF-7), is encoded by the FGF7 gene. KGF-1 only binds to the b splice form of the tyrosine kinase receptor, FGFR2b/KGFR. Affinity between KGF-1 and its receptor can be increased by heparin or heparan sulfate proteoglycan. FGF-10, also called keratinocyte growth factor 2 (KGF-2), shares 51% amino acid sequence identity and similar function to KGF-1, but uses an additional receptor, FGFR2c. KGF-1 plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. KGF-1 actives on keratinocytes, and exhibits mitogenic activity for epidermal cells, but essentially no activity for fibroblasts. KGF-1 has species cross active, human KGF-1 shares 96 % amino acid sequence identity with murine, and 92% with rat.