Melanoma Inhibitory Activity (MIA)

Inquiry

Cat Number

92576ES

If you have any other questions, please contact our experts.

Product Detail Description Scientific Support Customer Q&A

Storage

The products can be stored at -20℃ to -80℃ for 1 year.

Synonyms

CD-RAP

Molecular Weight

Approximately 12.1 kDa.

Appearance

Sterile filtered white lyophilized (freeze-dried) powder.

General Description

Melanoma inhibitory activity (MIA) is a low molecular weight soluble protein (~12 kDa) that is abundantly secreted by malignant melanoma cells as well as chondrocytes, but rarely expressed by normal skin cells. Although its name suggests it may play an inhibitory role in melanoma development, first characterized by inhibition of melanoma cell lines grown in vitro, it is instead highly pro-tumorigenic. It is a member of a protein family defined by a highly conserved SH3-like domain which mediates binding to several extracellular matrix components.

Mechanism of Action

The antitumor mechanism of MIA has been shown to be associated with increasing detachment and migration of cancer cells. This effect is achieved through specific binding of MIA to extracellular matrix proteins including fibronectin and laminin, as well as receptors expressed on the cell surface called integrins (α4β1, α5β1). The binding of MIA to these proteins conceals the underlying binding sites and physically inhibits melanoma cell attachment to their matrix. This detachment allows for migration of tumor cells into the blood and lymphatic systems, where they may form secondary tumors.

Application

MIA serves as a highly specific and sensitive serum biomarker for malignant melanoma. Because its levels in the blood correlate closely with the total tumor burden, it is used by oncologists to monitor disease progression and detect early relapse in patients after surgery. Beyond diagnostics, MIA is a significant target for therapeutic intervention. Researchers are developing small molecules and peptide inhibitors designed to block the MIA-integrin interaction, aiming to "anchor" cancer cells in place and prevent the spread of the disease.

MIA (melanoma inhibitory activity) orchestrates melanoma progression through a multifaceted mechanism: it enhances cell migration and invasion by inducing melanoma-associated genes like MT-MMP1, tPA, integrin β3, SPARC, and fibronectin while inhibiting anoikis (detachment-induced apoptosis); simultaneously, MIA suppresses melanocytic differentiation by downregulating pigment synthesis genes (tyrosinase, Trp-1) via transcriptional repression of MITF and PAX3 while promoting PIAS3 expression (a MITF inhibitor); this dual action disrupts cell-cell contacts via N-cadherin upregulation/E-cadherin downregulation, maintains an undifferentiated, invasive phenotype, and correlates with early tumor development. Consequently, MIA acts as a master regulator that drives melanoma progression by simultaneously promoting metastatic behavior and blocking differentiation, though its precise signaling pathways require further elucidation.

Fig. 1 Expression of melanoma inhibitory activity (MIA) in melanoma cell results in several cellular changes supporting melanoma development and progression. (Bosserhoff A K. 2005)

References

Bosserhoff A K. Melanoma inhibitory activity (MIA): an important molecule in melanoma development and progression. Pigment Cell Research. 2005, 18(6): 411-416.

What is the recommended long-term storage temperature for Melanoma Inhibitory Activity (MIA)?

Store lyophilized Melanoma Inhibitory Activity (MIA) at -20°C for maximum stability.

Should I avoid repeated freeze-thaw cycles for Melanoma Inhibitory Activity (MIA)?

Yes, freeze-thaw cycles degrade Melanoma Inhibitory Activity (MIA); please aliquot the solution before freezing.

How can I request a quote for Melanoma Inhibitory Activity (MIA)?

Simply click Inquiry on the Melanoma Inhibitory Activity (MIA) product page or email us.

How can I track the shipment of my Melanoma Inhibitory Activity (MIA)?

We provide a tracking number via email once your Melanoma Inhibitory Activity (MIA) leaves our facility.