IL-36RA-2

IL-1HY1, IL-1 delta, IL-1F5, IL-1H3, IL-1L1

Mouse interleukin-36 receptor antagonist (IL-36Ra; previously IL-1F5; also named FIL-1δ [delta], IL1HY1, IL-1H3, and IL-1L1) is a member of the IL1 family of proteins. IL1 family members include IL-1 beta, IL-1 alpha, IL-1ra, IL18 and IL1F5 through F10. All family members show a 12 beta -strand, beta -trefoil configuration, and are believed to have arisen from a common ancestral gene that underwent multiple duplications. The mouse IL36Ra/IL1F5 gene maps to a region on mouse chromosome 2 that contains all other IL-1 family members (except IL18), supporting an evolutionary relationship with the IL-1 family. It is particularly close to the gene for IL-1ra and is likely a relatively recent duplication of that gene. In humans, there is an alternate start site that potentially gives rise to an alternate splice form. This translated product has a premature stop codon, resulting in a truncated 16 aa peptide. Mouse to human, full length IL36Ra/IL1F5 has 90% aa identity. Within the family, IL-36Ra/IL-1F5 is 48%, 30%, 35%, 35%, 35%, 37% and 43% aa identical to IL1ra, IL1 beta, IL36 alpha /IL1F6, IL37/IL1F7, IL-36 beta /IL-1F8, IL36 gamma /IL1F9 and IL1F10, respectively. Cells reported to express IL-36Ra/IL-1F5 include monocytes, B cells, dendritic cells/Langerhans cells, keratinocytes, and gastric fundus Parietal and Chief cells. The receptor for IL-36Ra/IL-1F5 has not been positively identified. Indirect evidence suggests it is IL1 Rrp2 and/or IL-1 RAcP. In either case, activity association with receptor binding is unclear. It was initially reported to be an antagonist of IL-36 gamma activity. This would be consistent with its hypothesized relationship to IL1RA.

Approximately 16.9 kDa.

Sterile Filtered White lyophilized (freeze-dried) powder.

> 95% by SDS-PAGE and HPLC analyses.