Epigen

E.coli

EPG, Epithelial Mitogen

Epigen is an EGF-related polypeptide growth factor that signals through the ErbB receptor-1. It is produced in several tissues, including the testis, liver, and heart, as well as in certain tumor cells. Epigen is mitogenic for fibroblasts and epithelial cells. Human Epigen is initially synthesized as a glycosylated 14.7 kDa transmembrane precursor protein, which is processed by proteolytic cleavage to produce a mature soluble sequence. Recombinant Human Epigen is a 7.9 kDa monomeric protein, containing 72 amino acid residues, which comprises the EGF-homologous portion of the Epigen precursor.

Approximately 7.9 kDa

AVTVTPPITA QQADNIEGPI ALKFSHLCLE DHNSYCINGA CAFHHELEKA ICRCFTGYTG ERCEHLTLTS YA

Sterile Filtered White lyophilized (freeze-dried) powder.

> 98 % by SDS-PAGE and HPLC analyses.

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 300 ng/mL, corresponding to a specific activity of > 3.3 × 103 IU/mg.

< 1.0 EU per 1μg of the protein

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.