Cat Number: 92512ES

EMAP-II

Source

E.coli

Synonyms

SCYE1, EMAP-2, Small Inducible Cytokine Subfamily E Member 1

Description

SCYE1(small inducible cytokine subfamily E, member 1) is also named as EMAP2, AIMP1. The human cDNA encodes a 312-amino acid polypeptide that the predicted 34-kDa precursor can be cleaved to produce an active 20-kDa product(PMID:7929199). SCYE1 is secreted as a result of various stimuli, including TNF-α, heat shock, and hypoxia, and that it acts as a multifunctional cytokine on both endothelial and immune cells. Defects in AIMP1 are the cause of leukodystrophy hypomyelinating type 3 (HLD3). It has 2 isoforms produced by alternative splicing and the protein can exsit as a dimer.

Molecular Weight

Approximately 18.2 kDa

AA sequence

SKPIDVSRLD LRIGCIITAR KHPDADSLYV EEVDVGEIAP RTVVSGLVNH VPLEQMQNRM VILLCNLKPA KMRGVLSQAM VMCASSPEKI EILAPPNGSV PGDRITFDAF PGEPDKELNP KKKIWEQIQP DLHTNDECVA TYKGVPFEVK GKGVCRAQTM SNSGIK

Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

> 98% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by the apoptotic effect using serum free human MCF-7 cells is less than 40 ng/mL, corresponding to a specific activity of > 2.5 × 104 IU/mg.

Endotoxin

< 1.0 EU per 1μg of the protein

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

HOURS

Daily: 9.30 AM–6.00 PM
Sunday : 9.30 AM–1.00 PM
Holidays: Closed

INFORMATION