EG-VEGF-1

E.coli

Endocrine Gland-derived Vascular Endothelial Growth Factor, Prokineticin 1, PROK1

EG-VEGF is a secreted angiogenetic mitogen growth factor expressed in the steroidogenic glands, ovary, testis, adrenal gland, and placenta. EG-VEGF induces proliferation, migration, and fenestration (formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. The human EG-VEGF gene codes for a 105 amino acid polypeptide containing an N-terminal signal sequence of 19 amino acids. Recombinant Human EG-VEGF is a 9.6 kDa protein consisting of 86 amino acid residues, including ten cysteine residues that potentially form five pairs of intra-molecular disulfide bonds.

Approximately 9.7 kDa.

AVITGACERD VQCGAGTCCA ISLWLRGLRM CTPLGREGEE CHPGSHKVPF FRKRKHHTCP CLPNLLCSRF PDGRYRCSMD LKNINF

Sterile Filtered White lyophilized (freeze-dried) powder.

> 98 % by SDS-PAGE and HPLC analyses.

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using bovine EJG cells is less than 2.0 μg/mL, corresponding to a specific activity of > 500 IU/mg.

< 0.1 EU per 1μg of the protein

Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4, 0.02% Tween-20.