CXCL4-3

E.coli

chemokine (C-X-C motif) ligand 4, C-X-C motif chemokine 4, CXCL4, CXCL4iroplact, Iroplact, MGC138298, Oncostatin-A, PF4, platelet factor 4

CXCL4, also called PF4, is a small cytokine belonging to the CXC chemokine family and it is also known as chemokine (C-X-C motif) ligand. Mature mouse CXCL4 shares 76%, 88%, 64%, 64% and 63% amino acid sequence identity with human, rat, ovine, porcine and bovine CXCL4, respectively. Recombinant mouse CXCL4 contains 76 amino acids which is a single non-glycosylated polypeptide chain. CXCL4 can be antiproliferative and antiangiogenic, at least in part via interfering with FGF-2 and VEGF heparin binding and thus inhibiting their signaling. Tumor tissue revealed up-regulation of CXCL14 in cancer-associated fibroblasts of a majority of prostate cancer. Fibroblasts overexpressing CXCL14 promoted the growth of prostate cancer xenografts, and increased tumor angiogenesis and macrophage infiltration.

Approximately 9.5 kDa.

ESSFPATFVP LPADSEGGED EDLQCVCLKT TSGINPRHIS SLEVIGAGTH CPSPQLLATK KTGRKICLDQ QRPLYKKILK KLLDGDES

Sterile Filtered White lyophilized (freeze-dried) powder.

>95% by SDS-PAGE and HPLC analyses.

The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration of 10-100ng/ml. Fully biologically active when compared to standard.

< 0.1 EU per 1μg of the protein

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, 500 mM NaCl, pH 7.0.