Chemical Phosphorylation Reagent I

This reagent can be used to create the 3'-phosphate by adding it as the first addition to the support. After ammonia deprotection the oligo will have the 3'-phosphate attached to the 2nd base added during synthesis. Both the support base and the CPR are cleaved. The DMT-group is removed during the ammonium hydroxide deprotection and thus is not available for poly-pak purification.

656.77